Protein Purification Reagents and Kits
STEMCELL Technologies EasySep™ Mouse CD11c Positive Selection Kit II with Spleen Dissociation Medium, For processing: 2 x 10^9 cells
The EasySep™ Mouse CD11c Positive Selection Kit II isolates highly purified CD11c+ cells from splenocytes or other tissues by immunomagnetic positive selection. Desired cells are targeted with antibodies and magnetic particles, and isolated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Isolated cells are immediately ready for downstream applications such as flow cytometry, culture, and cell-based experiments.This product replaces the EasySep™ Mouse CD11c Positive Selection Kit (Catalog #18758) for even faster cell isolations and does not result in the labeling of isolated cells with PE.
STEMCELL Technologies EasySep™ Direct Human PBMC Isolation Kit RoboSep™, For processing: 100 mL whole blood processed
The EasySep™ Direct Human PBMC Isolation Kit is designed to isolate highly purified peripheral blood mononuclear cells (PBMCs) from fresh whole blood, buffy coat, bone marrow, cord blood, or leukapheresis products by negative selection. Red blood cells (RBCs), platelets, and unwanted cells are targeted for removal with antibody complexes and magnetic particles and separated using an EasySep™ magnet. Untouched PBMCs are simply collected into a new tube and are immediately available for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.
STEMCELL Technologies RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment Cocktail, For processing: 5000 mL cord blood processed
The RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment Kit is designed to pre-enrich hematopoietic progenitor cells from cord blood by negative selection. Unwanted cells are targeted for removal with antibody complexes recognizing CD3, CD11b, CD19 and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The hematopoietic progenitor cells are present as an enriched population at the interface between the plasma and the buoyant density medium. Cells of interest can then be positively selected from the pre-enriched cell population using EasySep™ (e.g. EasySep™ Human CD34 Positive Selection).
STEMCELL Technologies EasySep™ HLA Chimerism Whole Blood CD3 Positive Selection Kit, For processing: 60 mL buffy coat or whole blood processed
The EasySep™ HLA Chimerism Whole Blood CD3 Positive Selection Kit is designed to isolate CD3+ cells from fresh whole blood or buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD3 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD3 antigen is expressed on T cells and NKT cells.This product replaces the EasySep™ Human Whole Blood CD3 Positive Selection Kit (Catalog #18081) and EasySep™ HLA Whole Blood CD3 Positive Selection Kit (Catalog #18081HLA), for even faster cell isolations.
Hampton Research HR2-907-02/SaltRx 1 #02 (A2), 185 ml - 2.8 M Sodium acetate trihydrate pH 7.0, 0.1 M BIS-TRIS propane pH 7.0
HR2-907-02/SaltRx 1 #02 (A2), 185 ml - 2.8 M Sodium acetate trihydrate pH 7.0, 0.1 M BIS-TRIS propane pH 7.0. Please note items from this supplier are non-returnable.
Genscript Corporation Antibody Resin Protein Purification Protein Purification 5 ML 1/EA
GenScript Anti-DYKDDDDK Affinity Resin Easy (Cat. No. L00907) features high binding capacity specificity sensitivity stability and reusability making it particularly useful for the affinity purification and immunoprecipitation of DYKDDDDK-tagged fusion proteins expressed in common protein expression systems such as bacteria yeast and mammalian cells.
Hampton Research HR2-545/2.0 M Lithium sulfate monohydrate - 200 ml
HR2-545/2.0 M Lithium sulfate monohydrate - 200 ml. Please note items from this supplier are non-returnable.
STEMCELL Technologies RoboSep™ Human B Cell Enrichment Kit with Filter Tips, For processing: 1 x 10^9 cells
The EasySep™ Human B Cell Enrichment Kit is designed to isolate B cells from fresh or previously frozen peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-B cells and dextran-coated magnetic particles. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube. For even faster cell isolations, we recommend the new EasySep™ Human B Cell Isolation Kit (17954) (/products/easysep-human-b-cell-isolation-kit.html), which isolates cells in just 9 minutes.
STEMCELL Technologies RoboSep™ Human Progenitor Enrichment with Platelet Depletion, For processing: 1 x 10^9 cells
The EasySep™ Human Progenitor Cell Enrichment Kit with Platelet Depletion is designed to isolate hematopoietic progenitor cells from fresh or previously frozen cord blood and other cell preparations that contain large numbers of platelets by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD2, CD3, CD11b, CD11c, CD14, CD16, CD19, CD24, CD56, CD61, CD66b, glycophorin A and dextran-coated magnetic particles. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.
STEMCELL Technologies RosetteSep™ Human Total Lymphocyte Enrichment Cocktail, For processing: 200 mL whole blood processed
The RosetteSep™ Human Total Lymphocyte Enrichment Cocktail is designed to enrich lymphocytes from whole blood by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD16, CD36, CD66b and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as RosetteSep™ DM-L (Catalog #15705) or Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The purified lymphocytes are present as a highly enriched population at the interface between the plasma and the buoyant density medium.
STEMCELL Technologies RoboSep™ Human CD33 Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ Human CD33 Positive Selection Kit II is designed to isolate CD33+ cells from ammonium chloride lysed blood or bone marrow, or from nucleated cells prepared by density separation with RosetteSep™ DM-M density medium (Catalog #15725) by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD33 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD33 antigen is expressed on cells of the myeloid lineage.This product replaces the EasySep™ Human CD33 Positive Selection Kit (Catalog #18257) for even faster cell isolations.
STEMCELL Technologies RoboSep™ Human CD4+ T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Human CD4+ T Cell Isolation Kit is designed to isolate CD4+ T cells from fresh or previously frozen peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.This product can be used in place of the EasySep™ Human CD4+ T Cell Enrichment Kit (Catalog #19052) for even faster cell isolations.
Biotium Indo-1, AM Ester, 10x100 ug
Indo-1, AM ester is membrane-permeant and thus can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, Pluronic F-127, a mild detergent, is often used as a dispersing agent to facilitate cell loading. Indo-1/AM itself does not bind Ca2 , but it is readily hydrolyzed to Indo-1 by endogenous esterases once it enters cells. Properties: Light yellow solid soluble in DMSO; Store at -20°C and protect from light, especially in solution; C47H51N3O22; MW: 1010; [112926-02-0]
STEMCELL Technologies RoboSep™ Human Myeloid DC Enrichment Kit, For processing: 2 x 10^9 cells
The EasySep™ Human Myeloid DC Enrichment Kit is designed to isolate myeloid dendritic cells (mDC) from fresh peripheral blood mononuclear cells or ammonium chloride-lysed leukapheresis by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-mDCs and dextran-coated magnetic particles. The labeled cells are separated using the EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.